NidOil 100ml

Oil overlay for use during ICSI or gamete and embryo culture, inside or outside the incubator.

Components

• Light paraffin oil

Performance Characteristics

• Density: 0.82 – 0.88g/ml
• Endotoxin levels: <0.100 EU/ml
• Oil, bottles and stoppers are MEA tested

Features

• In vitro overlay for gametes and zygotes
• Maintains a constant environment within the culture medium
• Neither sticky nor overly viscous
• Sufficiently thick to prevent movement of the drops of media around the culture dish in order to facilitate pipetting
• Supplied in amber, screw-top bottles to prevent any light-induced changes (there have been several reports of paraffin oils becoming embryo-toxic after exposure to light on the laboratory bench)

Storage and Stability

• Store unopened bottles between 2 and 27 degrees Celsius. Avoid temperatures above or below these values. Expiry date is shown on both bottles and cartons.
• Open and close bottles under sterile conditions. After opening store between 2 and 8 degrees Celsius when not in use.
• NidOil has a shelf-life of 24 months from date of production when stored at recommended temperatures. Expiration dates on product labels apply only when the product is stored according to manufacturer’s recommendations.
• No antibiotics, unstable additives, or preservatives have been added to the product by the manufacturer.

Why is NidOil packed in amber bottles?

There have been several reports of other commercially available paraffin oils becoming embryo–toxic after exposure to light on the laboratory bench. Therefore, NidOil is packed in amber bottles as a precaution against light-induced changes to the product.

Should I equilibrate NidOil in the CO² incubator before use?

Yes, NidOil should be equilibrated in the same way as the culture medium before use to avoid differences in temperature and gaseous content between the components of the culture system.